American Journal of Microbiology and Immunology


Heamatologic Indices and Anti-Spermatogenic Effects of Dietary Supplemented Carica papaya seeds on Wister Rats

Review Article of American Journal of Microbiology and Immunology Heamatologic Indices and Anti-Spermatogenic Effects of Dietary Supplemented Carica papaya seeds on Wister Rats Ibeh Nnanna Isaiah1 ( BMLS, AMLSCN, MGHT),  Unuabonah Uyi Ibhoje2 ( BSC,MSc)  *1University of Benin Health Services Department, University of Benin PMB 1154 2University of Ibadan, Faculty of Life Science, Department of Microbiology The continuous usage of indegienious plants as an intervention tool for diseases that affect both humans and animals is as old as orthodox medicine in its self in this study we evaluated the overall effect of carica papaya seeds extract on wister rats to determine its heamatoligic stimulatory effect and the anti-spermatic effect. A total of eighty rats where orally administered the various doses of the extract over a period of time and the heamatoligic and spermatologic parameters where analyzed. From the results obtained there was a slight stimulatory effects as the total white blood cell increased according to the varying doses of 100mg and 200mg respectively (6.56±0.38 and 6.96±0.10) with mean packed cell volume of 38.0±0.84 and 37.4±1.03 also noticeable is the slight rise in the Lymphocytes from the control group to the varying doses of exposure 70±2.06 and 68.6±1.12, the total Sperm cell count across the varying doses where 11.6x106 +0.5cells/mm3 and 11.3x106 +0.5cells/mm3 respectively. The above showed a a little marked increase the heamatologic parameters there was a slight increase in the lymphocyte although there was a marked decrease of sperm cell count from the control down the doses of exposure. Keywords: Heamatologic, Anti-spermatogenic, Lymphocyte, White blood cell ...

Observed variations of Plasma Procalcitonin Amonsgt Sexually Active Males With Suspected Bacterial-Urogenital Infection In Yola Metropolis

Review Article of American Journal of Microbiology and Immunology Observed variations of Plasma Procalcitonin Amonsgt Sexually Active Males With Suspected Bacterial-Urogenital Infection In Yola Metropolis Ibeh N.Isaiah1, Omobogie E. NORAH 1Department of Health Services, University of Benin PMB 1154 Background: Male urogenital infection in Nigeria has increased due to alarming rise of unprotected sex amongst the sexually active males in Nigeria. Most of these case leads to a limiting advanced disease state such as kidney infections, bacteropsermia/infertility and reinal failure. AIM: To study the elevated serum Procalcitonin levels on patients with bacterial urogenital infections as an adjunct of bacterial-fungi-viral urogenital infections for diagnosis/prognosis and antimicrobial monitoring. PATIENTS and METHODS: The study group cut across adults from the ages of 20 years and above, 40 males who were suspected to have urogenital infection and 40 who were confirmed cases of urogenital infections those with suspected urogenital infections and those with confirmed urogenital infections. Using standard microbiological techniques urine and urethral swabs were taking and proper cultural techniques were carried out to isolate the bacterial/Fungi origin of infection, blood samples were collected from patients with suspected and confirmed urogenital infections for further analysis of the Procalcitonin ratio. RESULT: a total of 40 suspected males with signs and symptoms of urogenital infections where included in this study, a microbiological analysis was carried out on MSU(midstream urine clean catch) and urethral swab, and seminal fluid analysis, 40% yielded growth of gram Staphylococcus aureus and CoNs, 22.5% yielded growths of Escherichia coli, Proteus mirabilis, Acinectobacter spp, Klebseilla oxytoca while 15% had fungi/Parasitic origins,( Candida albicans and Trichomonas vaginalis) 22.5% showed no significant growth. The mean PCT ratio across the infectious agents were, bacterial origin ( 0.4-1.1 CI 0.34-0.62) fungi/parasitic origin (0.3-1.0 CI 0.28-0.56) Conclussion: although there was no remarkable difference between the confirmed cases of ...

Bacteruria Associated Urogenital Infections Amongst Women “a Study On Assymptomatic And Symptomatic Presentation In Benin Metropolis, Nigeria”.

Research Article of American Journal of Microbiology and Immunology Bacteruria Associated Urogenital Infections Amongst Women “a Study On Assymptomatic And Symptomatic Presentation In Benin Metropolis, Nigeria”.  IBEH.N.ISAIAH1 (BMLS, AIMLS, MNAAS) 1Department of Medical Microbiology, University of Benin Teaching Hospital, PMB 111 2Department of Microbiology, Faculty of Life Science, University Of Benin, PMB 1154, Introduction: Symptomatic and Asymptomatic urinary tract infection in women is of great importance to determine the causal and treatment of bacteruria in young women which may lead to disease states. Aim: to determine the occurrence of asymptomatic bacteriuria in young women and its relationship with urinary tract infection Methods: A microbiological analysis of urine was done on 3600 young women in urban Benin metroplolis from July 2009 to July 2010, a total of 1105 microorganism were isolated from both Symptomatic and Asymptomatic young women. Results: From the 1105 isolates from the descending order of occurrences Escherichia coli, Kleibseilla spp, Enterococcus faecalis and Staphylococcus aureus with Acinobacter spp with the least occurrence. Escherichia coli was the commonest cause of Asymptomatic urinary tract infection with 31.6 % and Acinectobacter sp 9.5% with the least occurrence of Asymptomatic urinary tract infections. Conclusion: This study shows that there is a rise in asymptomatic urinary tract infection amongst young women in Benin City. Keywords: BacterIuria, UTI, Asymptomatic ...

Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR

Research Article of American Journal of Microbiology and Immunology Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR S. M. El-Berbawy*; S.M. Sayed*;El-Toukhy, E. I. ** and Amal, A. Megahed*** * Assiut Lab. (Bacteriology Department) ** AHRI (Biotechnology Depart.) Dokki *** Port Said Lab. (Bacteriology Depart.) This study aims to investigate the slime production of Coagulase negative staphylococci (CoNS) isolates by phenotypic method on Congo Red Agar plates (CRA) and Genotypic detection of icaA, icaD and mecA genes by polymerase chain reaction (PCR). Out of 105 milk samples obtained from clinical bovine mastitis, 101samples (96.2%) were positive for bacterial growth. CoNS isolates was detected in 20 isolates with a percentage of 19.8%. Their ability to form biofilm as one of the most important virulence factors of the organisms using Congo Red Agar (CRA) method was investigated in which 13 out of 17 CoNS isolates (76.47%) were found to be slime producers. By PCR, mecA gene was found in threeout of 6 CoNS isolates (50%). Also six (100%) and three (50%) isolates were positive for icaA gene and icaD gene, respectively. In addition one isolate out of the six CoNS isolates (16.67%) was positive for the presence of icaA, icaD and mecA genes and also has the ability to form biofilm. The in vitro activities of CoNS against 11 selected antimicrobial agents referred that the highest resistance rate of CoNS observed to Lincomycin (100%), followed by Cefotaxime (94.41%), Oxacillin (58.82%), Ampicillin (47.06%) and Penicillin (41.18%), while the highest rate of sensitivity observed to Enrofloxacin and Gentamicin (100%, for each), followed by Doxycycline (94.11%).Conclusion, the findings of the present study demonstrated the ability of CoNS isolated from bovine clinical mastitis to form biofilms. This must be considered as an alarming situation, ...

The Accumulation Of Cadmium In Corn At Different Levels Of Soil Ph

Research Article of American Journal of Microbiology and Immunology The Accumulation Of Cadmium In Corn (Zea Mays L.) At Different Levels Of Soil Ph Rabah S. Shareef*1,2, Awang Soh Mamat*3, Zakaria Wahab*4, Ibni Hajar Rukunudin*5 1 College of Education-Al-Qiam, University of Anbar, Iraq. 2 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 3 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 4 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 5 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. A pot experiment was carried out in the plastic greenhouse at the Institute of Sustainable Agrotechnology (INSAT), University Malaysia Perlis in Sungai Chuchuh, Perlis, Malaysia. During season of the cultivation of 2014 investigate the impact of soil pH and cadmium on growth of corn plant (Zea mays L.). Twenty five were arranged in factorial experiments according to the Complete Randomized Design (CRD), with three replicates. Five levels of soil pH were, pH4, pH5.2 (i.e., the original value), pH6, pH7 and pH8 and five levels of cadmium (Cd); Cd 1, Cd 2, Cd 3, Cd 4 and Cd 0 where the amounts (2, 4, 6, 8 mg. kg-1 soil and control treatment without add cadmium) are applied as CdCl2. Thus, the total numbers of pots were 75 pots. The results of this investigation revealed that; The decrease of soil pH led to significant effect to increase concentration of cadmium in root, stem, leaves and grain of corn, where the level of pH 5.2 gave highest concentrations of Cd compared with pH 8. the interactions between soil pH and cadmium led to increase of reduced the accumulation of cadmium in the corn, where it gave the interaction between pH 8 and Cd 0 lowest concentration of cadmium in root, stem, leaves and grain. Keywords: Accumulation, Cadmium, Corn, Soil pH, ...

Dr. Aziza Mahrous Mohamed Amer
Dean and Professor of pharmacology Department of Pharmacology – Faculty of Veterinary Medicine, Cairo University.; Fellow of National Academy of Science and Technology 2016; Member of Drug Council Academy of Science and Technology 2016

Dr. Pongsak Rattanachaikunsopon
Professor, Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand

Dr. Ying-Yu Jin
Head of Fermentation Team, Center for Nutraceutical and Pharmaceutical Materials, Yongin, Korea; Research Professor, Department of Biomodulation, Myongji University, Yongin, Korea.

Dr. Helen Treichel
Professor, Researcher, Deputy Coordinator of Research and Graduate Studies and Coordinator of the Graduate Program, Environmental Science and Technology at Universidade Federal da Fronteira Sul (UFFS) – Campus Erechim

Dr. Liesel Brenda Gende
Professor of Food Microbiology and Food Chemistry, Department of Chemistry, Faculty of Natural Science, Mar del Plata University, Buenos Aires-Argentina. Researcher at CONICET (National Council of Scientific and Technical Research of Argentina). Fellow of the Research Center on Social Bees. Specialty in apicultural microbiology.

Dr. Akrum Hamdy 
Prof. Poultry Physiology, Department Head of Animal and Poultry Production, Faculty of Agriculture, Minia University, Egypt, Fellow Academy Scientific Research and Technology

Dr. Mohamed Hamed Mohamed Al-Agamy
Professor, Microbiology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Dr. Aditi Singh
Associate Professor, Amity Institute of Biotechnology, Amity Univesity Uttar Pradesh, Lucknow Campus, Malhaur, Gomti Nagar Extension, Lucknow-226028.

Dr. Kantha Deivi Arunachalam 
Professor and Head, Centre for Environmental Nuclear Research, Directorate of Research & Virtual Education, SRM University, Chennai, Tamilnadu., India.

Profa. Dra. Fabiana América Souza 
Universidade de Pernambuco – UPE (Campus Mata Norte), Universidade Federal Rural de Pernambuco – UFRPE, Universidade do Minho – UMinho/Portugal, Fundação de Ensino Superior de Olinda – FUNESO.

Dr.  Parichat Phumkhachorn
Assistant Professor, Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, THAILAND

Dr. Bamidele RAHEEM 
Researcher, Department of Food and Environmental Sciences, University of Helsinki, Finland.

Dr. Iran ALEMZADEH
Professor, Chemical and Petroleum Engineering Department, Sharif University of Technology, P.O. Box 11155-9468, Tehran IRAN.

Dr. Younes Smani
Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, Infectious Diseases Research Group, University of Seville/CSIC/University Hospitals Virgen del Rocio and Virgen Macarena, Seville, Spain.

Dr. Surabhi Mishra
Resarch Scientist, Dept. of Microbiology, University of Iowa, IA – 52242

Dr. Elsayed Ibrahim Elagamy
Professor of Immunology, Department of Applied Medical Sciences, College of Community (Unaizah), Qassim University, Kingdom of Saudi Arabia.

Manuscript Title: The title should be a brief phrase.

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Abstract: The abstract should be less than 500 words. Following abstract, a list of keywords and abbreviations should be added. The keywords should be no more than 10. Abbreviation are only used for non standard and long terms.

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References: References should be listed in a numbered citation order at the end of the manuscript. DOIs and links to referenced articles should be added if available. Abstracts and talks for conferences or papers not yet accepted should not be cited. Examples Published Papers:

1.Ibeh Isaiah Nnanna. Elevated Serum Procalcitonin an Adjunct for Early detection of Infant Tuberculosis in Paediatric HIV/AIDS. American Journal of Microbiology and Immunology, 2016,1:8. DOI: 10.28933/nnanna-ajmi-10-2016 

2.Jamal Bayed Salim.,Ahmed K. Bolad, Hamid Suliman A.. andss Mujeeb A.Kabbashi. The effect of Transfer Factor as Immunotherapy comparing with the effect of BCG in Mice challenged with Mycobacterium tuberculosis. American Journal of Microbiology and Immunology, 2017; 2:1. DOI: 10.28933/ajmi-2017-02-2801 

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American Journal of Microbiology and Immunology

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