HEAMATOLOGY AND SERUM BIOCHEMICAL INDICES OF GROWING RABBITS FED DIET SUPPLEMENTED WITH DIFFERENT LEVEL OF INDIGOFERA ZOLLINGERIANA LEAF MEAL


HEAMATOLOGY AND SERUM BIOCHEMICAL INDICES OF GROWING RABBITS FED DIET SUPPLEMENTED WITH DIFFERENT LEVEL OF INDIGOFERA ZOLLINGERIANA LEAF MEAL


Alagbe, J.Oa and Oluwafemi, R.Ab

aDepartment of Animal Nutrition, Sumitra Research Farm, Gujarat, India.
bDepartment of Animal Science, Faculty of Agriculture, University of Abuja. Nigeria


International Journal of Animal Research

This study was conducted to determine the hematological and serum biochemical parameters of growing rabbits fed diet supplemented with Indigofera zollingeriana leaf meal (IZM). A total number of Fifty (50) growing rabbits of between seven and eight weeks of age were randomly assigned to five treatment groups in a completely randomized design. Each group was replicated five times with two rabbits per replicate. Five experimental diets were formulated such that T1, T2, T3, T4 and T5 was supplemented with IZM at 0g, 2.5g, 5.0g, 7.5g and 10g respectively. Feed and water were provided ad libitum and the experiment lasted for 8 weeks. The hematological parameters determined included pack cell volume (PCV), hemoglobin (Hb), red blood cells (RBC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), white blood cell (WBC) and its differentials while those of serum biochemical indices are: total protein, globulin, albumin, cholesterol, serum glutamic pyruvate transaminase (SGPT), serum glutamic oxaloacetate transaminase (SGOT). Results on hematology revealed that there were no significant differences (P>0.05) in the values of PCV, RBC, MCV, WBC and its differentials. Hb, MCH and MCHC values were significantly (P<0.05) influenced by the dietary supplementation of IZM. All results on the serum biochemical parameters showed that there was no significant (P>0.05) differences among the treatments. It was concluded that dietary supplementation of IZM up to 10g/kg had no deleterious effect on the hematology and serum biochemical parameters of rabbits.


Keywords: Growing rabbits; hematology, serum biochemical parameters; Indigofera zollingeriana

Free Full-text PDF


How to cite this article:
Alagbe, J.O and Oluwafemi, R.A, HEAMATOLOGY AND SERUM BIOCHEMICAL INDICES OF GROWING RABBITS FED DIET SUPPLEMENTED WITH DIFFERENT LEVEL OF INDIGOFERA ZOLLINGERIANA LEAF MEAL. International Journal of Animal Research, 2019; 4:28.


References:

1. AOAC (1999). Official methods of analysis, 2nd edition. Association of analytical chemists. Washington, D.C.
2. Abdullah, L., D. Apriastuti & T. Apdini (2017). Use of Indigofera zollingeriana as Forage Protein Source in Dairy Goat Ration.Bogor Agricultural University Indonesia.
3. Ali, A., Abdullahi, L, Karti, PDMH, Chozin, M.A and Astuti, D.A (2014). Production and Nutritive value of Indigofera zollingeriana and Leucaena leucocephala in Peatland. A Ali et al/Production 16(3):156-164, September 2014. DGHE No.81/DIKTI/Kep./2011.1411-2027.
4. Ayodeji Adedirea, Olutosin Oduguwa, Vincent Jegede (2013). Performance and Haematology of Weaner Rabbits Fed Diets Containing Culture Fermented Cowpea Husk. Tropentag 2013, Stuttgart, Germany September 17-19, 2013.
5. A.O.A.C (2000). Association of Official Analytical Chemists. Official Methods of Analysis 19th Edition Washington, D.C Pp69-77.
6. Archetti I., Tittarelli C., Cerioli M., Brivio R., Grilli G., Lavazza A. 2008. Serum chemistry and hematology values in commercial rabbits: preliminary data from industrial farms in northern Italy. In proc.: 9th World Rabbit Congress, 10-13 June, Verona, Italy, 1147-1151.
7. Ahemen, T., A. H. Abu and V. Gbor (2013). Haematological and serum biochemical parameters of rabbits fed varying dietary levels of water spinach (Ipomoea aquatic) leaf meal. Advances in Applied Science Research, 2013, 4(2):370-373.
8. Bush, B.M (1991). Interpretation of Laboratory Results for Small Animal Clinicals. Blackwell scientific publication London.
9. Byanet, O., Adamu, S, Salami, S.O and Obadiah, H. I (2008). Heamatological and plasma biochemical parameters of young grass cutters reared in the Northern Nigeria. J. of cell and animal biology. Vol 2(10), pp 177-181.
10. Brugger, D and Wilhelm, M.W (2015). Environmental responsibilities of livestock feeding using trace mineral supplements. Review Animal Nutrition. 1:113-118.
11. Cowan, M. M (1999). Plant products as antimicrobial agents. Clinical Micr. Rev., 12(4): 564-582.
12. Chandrasekaran Swaminathan (2018). Phytochemical analysis and antibacterial and antioxidant properties of Indofera tinctoria. Asian J. Pharm and Clinical Res. Vol 11, Issue 6, 2018.
13. Dahot, M.U (1999). Antibacterial and antifungal activity of protein of Indigofera leaves. J. Ethnopharmacol., 64(3):277-282.
14. Elmas M., Yazar E., Uney K., Karabacak A. 2006. Pharmacokinetics of flunixin after intravenous administration in healthy and endotoxaemic rabbits. Vet Res Commun. 30:73-81. doi: 10.1007/s11259-005-3227-7
15. Jenkins, J.R (2008) Rabbit diagnostic testing .J. Exot. Pet Med., 17:4-15.
16. Jiwuba, P.C., K. Ikwunz, E. Dauda and D. O. Ugwu (2016). Haematological and Serum Biochemical Indices of Growing Rabbits Fed Diets Containing Varying Levels of Moringa oleifera Leaf Meal. British Biotechnology Journal. 15(2): 1-7, 2016.
17. Ladipo M. K., Adu O. A., Oyefeso S., Akinmuyisitan I. W (2015). Growth Performance and Blood Profile of Male Rabbits Fed Dietary Cerium Oxide. International Journal of Agriculture, Forestry and Fisheries 2015; 3(3): 87-92.
18. Taiwo A. Abidemi., Oyedapo J. Adebayo, Olwadare Idowu and M.O. Agbotoba (2009). Nutrient content and anti-nutritional factors in shea butter (Butryospermum parkii) leaves. African Journal of Biotechnology. Vol 8(21) pages 5888-5890.
19. National Research Council (1977). Nutrients requirements for rabbits, second edition. National Academy of Science, Washington D.C pages 10-
20. Roschian, L., Bernat, E and Grubber, W (1974).Enzmtrache Bestimung des gesamcholestrins in serus. l. clin. Chein. Bioc/iem. 12:403-407.
21. Statistical Analysis Systems Institute Inc. (2008). SAS User guide statistic, version 6 edition. Gary, New York, USA.
22. Harbone, I. B (1973) A guide to modern techniques to plant analysis. Chapman and hall, New York, USA 2nd Edition.
23. Sofowora, A. (1993). Medicinal plants and Traditional Medicine in Africa. Spectrum Books Limited Ibadan. Nigeria. P 289.
24. Jain, N.C (1986) Schalms veterinary hematology 4th edition Lea and Febiger, Philadelphia
25. Odebiyi, A and Sofowora, A. E (1978). Phytochemical screening of Nigerian medicinal plant. Part III Lloydia, 41, 234-246.
26. WHO (1996). Trace elements and other essential nutrients. Clinical Application of Tissue Mineral Analysis 2nd edition, Trace elements USA, ISBN 13:9781885676153, Pages 182.
27. Murray, R. K., Granner, D.K, Mayes, P.A and Rodwell, V. W (2000). Harper’s Biochemistry, 25th edition, McGraw-Hill, Health Profession Division, USA.
28. Munro O. Bansir (1969). Oxalate in Nigerian vegetables. West African J. Bio. Appl. Chem. 12:14-18.
29. Ozcan, M (2003). Mineral contents of some plants used as condiments in Turkey. Food Chemistry 84:437-440.
30. Vikas Kumar., Amit K. Sinha, Harinder P.S. Makka, Klaus Becker (2009). Dietary roles of phytate and phytase in human nutrition: A review. Food Chemistry 120 (2010) 945–959.
31. Musa, A.M., Abbas, G and Aliyu, A.B (2008). Phytochemical and antimicrobial screening of Indigofera conferta. Research J. of Medicinal Plant. 2(2):74-78, 2008.
32. Taj Ur Rahman, Mohammad Aurang Zeb, Wajiha Liaqat, Mohammad Sajid, Sajjad Hussain and M. Iqbal Choudhary (2018). Phytochemistry and pharmacology of genus Indigofera: A review. Rac. Nat. Prod. 12: 1(2018) 1-13.
33. Wright, K (2002). Healing foods, Geddes and Grosset, Scotland Pages 8-31
34. Poole, T.B (1987). The UFAW Handbook on care and management of laboratory and other research animals. (6th edn). Universities Foundation for Animal Welfare, Longman Scientific and Technical, Harlow, U.K.
35. Isaac, L. J., Abah, G., Akpan, B and Ekaette, I.U (2013) Haematological Properties of Different Breeds and Sexes of Rabbits (p.24-27). Proceedings of the 18th Annual conference of Animal Association of Nigeria
36. Özkan C., Kaya A., Akgül Y (2012). Normal values of haematological and some biochemical parameters in serum and urine of New Zealand White rabbits. World Rabbit Sci. 2012, 20: 253 – 259. doi:10.4995/wrs.2012.1229
37. Tavares F.L., Sousa-e-Silva M.C.C., Santoro M.L., Barbaro K.C., Rebecchi I.M.M., Sano-Martins I.S. 2004. Changes in hematological, hemostatic and biochemical parameters induced experimentally in rabbits by Loxosceles gaucho spider venom. Hum. Exp. Toxicol., 23: 477-486. doi: 10.1191/0960327104ht475oa
38. Silva T.D.O., Kreutz L.C., Barcellos L.J.G., Borella J., Soso A.B., Souza C. 2005. Reference values for chinchilla (Chinchilla laniger) blood cells and serum biochemical parameters. Cienc. Rural, 35: 602-606.
39. Opara, C. C (1996) Studies on the use of Alchornia ordifolia leaf meal as feed ingredient in poultry diets. M.Sc thesis, federal University of technology, Owerri, Nigeria. Pp 150-159.
40. Maikai, V.A, Abubakar, U., Salman, A.A and Inuwa, T.N (2010). Preliminary survey of medicinal plants used in the treatment of trypanosomaisis in Kaduna State, Nigeria. Ethnobotanical leaflets 2010, 14:319-326.