Research Article of International Journal of Bioscience and Medicine
Response surface methodology for the optimization of chlorpyrifos-degrading conditions by Pseudomonas stutzeri ZH-1
Feng HE 1, Mi-mi ZHANG 2, Li-hong ZHANG1 and Qing-ping HU 1*
1College of Life Science, Shanxi Normal University, Linfen, China
2Modern College of Humanities Sciences, Shanxi Normal University, Linfen, China
The removal of pesticides in the environment mainly depends on natural degradation, especially on microbial degradation. Biodegradation has many advantages, such as complete degradation, no secondary pollution, quick effect and wide spectrum. Based on the single-factor experiments and Box-Benhnken design, the effect of four factors on the degradation of chlorpyrifos by P. stutzeri ZH-1 was investigated. The four factors, including temperature (°C) , oscillator speed (rpm), inoculum concentration (%) and pH, and their interactions on the degradation of chlorpyrifos were studied through the use of response surface analysis.The optimal conditions of chlorpyrifos-degrading were as follows: temperature 36.7°C, oscillator speed 130.00rpm, inoculum concentration 7%, pH 7. Under these conditions,the degradation rate of chlorpyrifos was 96.48%. Moreover, P. stutzeri ZH-1 could be used efficiently for remediation of contaminated soils.
Keywords: response surface methodology; chlorpyrifos-degrading; Pseudomonas stutzeri ZH-1
How to cite this article:
Feng HE, Mi-mi ZHANG, Li-hong ZHANG and Qing-ping HU. Response surface methodology for the optimization of chlorpyrifos-degrading conditions by Pseudomonas stutzeri ZH-1. International Journal of Bioscience and Medicine, 2018; 2:7. DOI: 10.28933/ijbm-2018-01-0801
 Mariusz C, Marcin W, Zofia P. Biodegradation of the organophosphorus insecticide diazinon by Serratia sp. and Pseudomonas sp. and their use in bioremediation of contaminated soil. Chemosphere,2009, (76) :494–501.
 Li XH, Jiang JD, Gu LF,et al. Diversity of chlorpyrifos-degrading bacteria isolated
from chlorpyrifos-contaminated samples.International Biodeterioration and Biodegradation,2008, (62): 331–335.
 Singh BK, Walk A, Morgan JAW, et al. Effects of soil pH on the biodegradation of chlorpyrifos and isolation of a chlorpyrifos-degrading bacterium. Applied and Environmental Microbiology, 2003, 69(12) :5198-5206.
 Singha BK, Walkera A, Wright,DJ. Bioremedial potential of fenamiphos and chlorpyrifos degrading isolates: Influence of different environmental conditions.Soil Biology and Biochemistry, 2006,(38): 2682–2693.
 Mallick K, Bharati K, Banerji A, et al. Bacterial degradation of chlorpyrifos in pure cultures and in soil. Bulletin of Environmental Contamination and Toxicology, 1999, 62(1): 48-54.
 Singh BK, Walker A, Morgan JAW, et al. Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Applied and Environmental Microbiology, 2004, 70(8): 4855-4863.
 Shi YH.Isolation and identification of pesticides-degrading bacterium Pseudomonas stutzeri YC-YH1 and study of degradation mechanism. 2012.
 Holden, P.A., Firestone, M.K. Soil microorganisms in soil cleanup:how can we improve our understanding? Journal of Environmental quality ,1997,26, 32–40.
 Vidali, M. Bioremediation. An overview. Pure Applied Chemistry,2001,73, 1163–1172.
 Chen K, Liu XM, Li R, et al.Isolation of a buprofezin co-metabolizing strain of Pseudomonas sp. DFS35-4 and identification of the buprofezin transformation pathway.Biodegradation,2011,( 22):1135–1142.
 Abo-Amer, Aly E.Biodegradation of Diazinon by Serratia marcescens DI101 and its Use in Bioremediation of Contaminated Environment. Journal of Microbiology and Biotechnology,2011, 21(1):71–80.
 Ramkrishna S, Swaminathan T. Response surface modeling and optimization to elucidate and analyze the effects of inoculum age and size on surfactin production.Biochemical Engineering Journal, 2004, (21) :141–148.
 Dhanya G, Swetha S, Ashok Pa et al.Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens. Bioresource Technology, 2008, (99): 4597–4602.
 Mahiudddin M, Fakhruddin ANM, Abdullah, et al. Degradation of the organophosphorus insecticide diazinon by soil bacterial isolate. The International Journal of Biotechnology, 2014, 3(1): 12-23.
 Sikora L J, Kaufman D, and Hornog L C. Enzyme activity in soils showing degradation of organophosphosphate insecticides. Biology and Fertility of Soils，1990, 9(1): 14-18.
 Racke K D, Robbins S T. Factors affecting the degradation of 3,5,6-trichloro-2-pyridinol in soil. ACS Symposium Series, 1991, 459: 93-107.
 Yang L，ZhaoYH and Zhang BX,et al.Isolation and characterization of a chlorpyrifos degrading bacteria and its bioremediation application in the soil. Acta Microbiologica Sinica, 2005,45(6): 905-909.
 Zhang DY,Tan XQ and Luo XW,et al.Isolation of photosynthetic bacteria HP-1with degradation of organic-phosphorus insecticides and studies on its biodegradation ability and capacity of increasing growth.Life Science Research,2005, 9(3): 247-253.
 Xu G, Zheng W, Li Y, et al. Biodegradation of chlorpyrifos and 3,5,6-Trichloro-2-Pyridinol by a newly solated Paracoccus sp. strain RP. International Biodeterioration and Biodegradation, 2008, 62(1): 51-56.
 Sasikala C, Jiwal S, Rout P, et al. Biodegradation of chlorpyrifos by bacterial consortium isolated from agriculture soil. World Journal of Microbiology and Biotechnology, 2012, 28(3): 1301-1308.